List of Parasporin-Related Publications

  1. Mizuki, E., Ohba, M., Akao, T., Yamashita, S., Saitoh, H. and Park, Y.S. Unique activity associated with non-insecticidal Bacillus thuringiensis parasporal inclusions: in vitro cell-killing action on human cancer cells. J. Appl. Microbiol., 86, 477-486 (1999). FreePDF
  2. Kim, H.-S., Yamashita, S., Akao, T., Saitoh, H., Higuchi, K., Park, Y. S., Mizuki, E. and Ohba, M. In vitro cytotoxicity of non-Cyt inclusion proteins of a Bacillus thuringiensis isolate against human cells, including cancer cells. J. Appl. Microbiol., 89, 16-23 (2000). FreePDF
  3. Lee, D.-W., Akao, T., Yamashita, S., Katayama, H., Maeda, M., Saitoh, H., Mizuki, E. and Ohba, M. Noninsecticidal parasporal proteins of a Bacillus thuringiensis serovar shandongiensis isolate exhibit a preferential cytotoxicity against human leukemic T cells. Biochem. Biophys. Res. Commun., 272, 218-223 (2000). Link to PubMed
  4. Mizuki, E., Park, Y. S., Saitoh, H., Yamashita, S., Akao, T., Higuchi, K. and Ohba, M. Parasporin, a human leukemic cell-recognizing parasporal protein of Bacillus thuringiensis. Clin. Diagn. Lab. Immunol., 7, 625-634 (2000). FreePDF
  5. Yamashita, S., Akao, T., Mizuki, E., Saitoh, H., Higuchi, K., Park, Y. S., Kim, H.-S. and Ohba, M. Characterization of the anti-cancer-cell parasporal proteins of a Bacillus thuringiensis isolate. Can. J. Microbiol., 46, 913-919 (2000). Link to PubMed
  6. Lee, D.-W., Katayama, H., Akao, T., Maeda, M., Tanaka, R., Yamashita, S., Saitoh, H., Mizuki, E. and Ohba, M. A 28-kDa protein of the Bacillus thuringiensis serovar shandongiensis isolate 89-T-34-22 induces a human leukemic cell-specific cytotoxicity. Biochim. Biophys. Acta., 1547, 57-63 (2001). Link to PubMed
  7. Yamagiwa, M., Namba, A., Akao, T., Mizuki, E., Ohba, M. and Sakai, H. Cytotoxicity of Bacillus thuringiensis crystal protein against mammalian cells. Mem. Fac. Eng. Okayama, University, 36, 61-66 (2002).
  8. Namba, A.,Yamagiwa M., Amano, H., Akao, T., Mizuki, E., Ohba, M. and Sakai, H. The cytotoxicity of Bacillus thuringiensis subsp. coreanensis A1519 strain against the human leukemic T cell. Biochim. Biophys. Acta., 1622, 29-35 (2003). Link to PubMed
  9. Ito, A., Sasaguri, Y., Kitada, S., Kusaka, Y., Kuwano, K., Masutomi, K., Mizuki, E., Akao, T., Ohba, M. Selective cytocidal action of a crystal protein of Bacillus thuringiensis on human cancer cells. J. Biol. Chem., 279, 21282-21286 (2004). FreePDF
  10. Okumura, S., Akao, T., Higuchi1, K., Saitoh, H., Mizuki,E., Ohba, M. and Inouye, K. Bacillus thuringiensis serovar shandongiensis strain 89-T-34-22 produces multiple cytotoxic proteins with similar molecular masses against human cancer cell. Lett. Appl. Microbiol., 39, 89-92 (2004). FreePDF
  11. Akiba, T., Abe, Y., Kitada, S., Kusaka, Y., Ito, A., Ichimatsu, T., Katayama, H., Akao, T., Higuchi, K., Mizuki, E., Ohba, M., Kanai, R. and Harata, K. Crystallization of parasporin-2, a Bacillus thuringiensis crystal protein with selective cytocidal activity against human cells. Acta Cryst. D60, 2355-2357 (2004). Link to PubMed
  12. Katayama, H., Yokota, H., Akao, T., Nakamura, O., Ohba, M., Mekada, E. and Mizuki, E. Parasporin-1, a novel cytotoxic protein to human cells from non-insecticidal parasporal inclusions of Bacillus thuringiensis. J. Biochem., 137, 17-25 (2005). Link to PubMed
  13. Yamashita, S., Katayama, H., Saitoh, H., Akao, T., Park, Y.S., Mizuki, E., Ohba, M. and Ito, A. Typical three-domain Cry proteins of Bacillus thuringiensis strain A1462 exhibit cytocidal activity on limited human cancer cells. J. Biochem., 138, 663 - 672 (2005). Link to PubMed
  14. Okumura, S., Saitoh, H., Ishikawa,T., Wasano, N., Yamashita, S., Kusumoto, K., Akao, T., Mizuki, E., Ohba, M. and Inouye, K. Identification of a Novel Cytotoxic Protein, Cry45Aa, from Bacillus thuringiensis A1470 and Its Selective Cytotoxic Activity against Various Mammalian Cell Lines. J. Agric. Food Chem., 53, 6313-6318 (2005). Link to PubMed
  15. Amano, H., Yamagiwa, M., Akao, T., Mizuki, E., Ohba, M. and Sakai, H. A novel 29-kDa crystal protein from Bacillus thuringiensis induces caspase activation and cell death of Jurkat T cells. Biosci. Biotechnol. Biochem., 69, 2063-2072 (2005). FreePDF
  16. Saitoh, H., Okumura, S., Ishikawa, T., Akao, T., Mizuki, E. and Ohba, M. Investigation of a novel Bacillus thuringiensis gene encoding a parasporal protein, parasporin-4, that preferentially kills leukaemic T cells. Biosci. Biotechnol. Biochem., 70, 2935-2941 (2006). FreePDF
  17. Okumura, S., Saitoh, H., Wasano, N., Katayama, H., Higuchi, K., Mizuki, E. and Inouye, K. Efficient Solubilization, activation and purification of recombinant Cry45Aa of Bacillus thuringiensis expressed as an Escherichia coli inclusion body. Protein Expres. Purif. 47, 144-151 (2006). Link to PubMed
  18. Yasutake, K., Binh, N.D., Kagoshima, K., Uemori, A., Ohgushi, A., Maeda, M., Mizuki, E., Yu, Y.M. and Ohba, M. Occurrence of parasporin-producing Bacillus thuringiensis in Vietnam. Can. J. Microbiol., 52, 365-372 (2006). Link to PubMed
  19. Akiba, T., Higuchi, K., Mizuki, E., Ekino, K., Shin, T., Ohba, M., Kanai, R. and Harata, K. Nontoxic crystal protein from Bacillus thuringiensis demonstrates a remarkable structural similarity to beta-pore-forming toxins. Proteins, 63, 243-248 (2006). FreePDF
  20. Kitada, S., Abe, Y., Shimada, H., Kusaka, Y., Matsuo, Y., Katayama, H., Okumura, S., Akao, T., Mizuki, E., Kuge, O., Sasaguri, Y., Ohba, M. and Ito, A. Cytocidal Actions of Parasporin-2, an Anti-tumor Crystal Toxin from Bacillus thuringiensis. J. Biol. Chem., 281, 26350-26360 (2006).
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  22. Uemori, A., Maeda, M., Yasutake, K., Ohgushi, A., Kagoshima, K., Mizuki, E. and Ohba, M. Ubiquity of parasporin-1 producers in Bacillus thuringiensis natural populations of Japan. Naturwissenschaften, 94, 34-38 (2007). Link to PubMed
  23. Katayama, H., Kusaka, Y., Yokota, H., Akao, T., Kojima, M., Nakamura, O., Mekada, E. and Mizuki, E. Parasporin-1, a novel cytotoxic protein from Bacillus thuringiensis, induces Ca2+ influx and a sustained elevation of the cytoplasmic Ca2+ concentration in toxin-sensitive cells.J. Biol. Chem., 282, 7742-7752 (2007). FreePDF
  24. Jung, Y.-C., Mizuki, E., Akao, T. and Cote, J.-C. Isolation and Characterization of a Novel Bacillus thuringiensis Strain Expressing a Novel Crystal Protein with Cytocidal Activity Against Human Cancer Cells. J. Appl. Microbiol. 103, 65-79 (2007).
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  26. Ichikawa, M., Uemori, A., Ohgushi, A., Yasutake, K., Kagoshima, K., Mizuki, E., Binh, N.D. and Ohba, M. Phenotypic and morphometric characterization of Bacillus thuringiensis parasporin-1 producers: a comparison with insecticidal Cry-protein producers. J. Fac. Agr., Kyushu Univ., 52, 307-313 (2007).
  27. FreePDF
  28. Hayakawa, T., Kanagawa, R., Kotani, Y., Kimura, M., Yamagiwa, M., Yamane, Y., Takebe, S. and Sakai, H. Parasporin-2Ab, a newly isolated cytotoxic crystal protein from Bacillus thuringiensis. Curr Microbiol. 55, 278-83 (2007). Link to PubMed
  29. Yasutake, K., Uemori, A. Binh, N.D., Mizuki, E. and Ohba, M. Identification of parasporin genes in Vietnamese isolates of Bacillus thuringiensis. Zeitschrift fur Naturforschung C., 63, 139-143 (2008). Link to PubMed
  30. Uemori, A., Ohgushi, A., Yasutake, K., Maeda, M., Mizuki, E. and Ohba, M. Parasporin-1Ab, a Novel Bacillus thuringiensis Cytotoxin Preferentially Active on Human Cancer Cells In Vitro. Anticancer Research, 28, 91-95 (2008). Link to PubMed
  31. Inouye, K., Okumura, S. and Mizuki, E. Parasporin-4, A Novel Cancer Cell-killing Protein Produced by Bacillus thuringiensis. Food Science and Biotechnology, 17, 219-227 (2008).
  32. Ichikawa, M., Uemori, A., Yasutake, K., Mizuki, E. and Ohba, M. Failure to phenotypically discriminate between non-insecticidal Bacillus thuringiensis strains with anticancer parasporins (PS2, PS3, and PS4) and Bacillus thuringiensis strains that produce insecticidal Cry proteins. Applied Entomology and Zoology, 43, 421-426 (2008).
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  34. Nadarajah, V.D., Ting, D., Chan, K.K., Mohamed, S.M., Kanakeswary, K. and Lee, H.L. Selective cytotoxic activity against leukemic cell lines from mosquitocidal Bacillus thuringiensis parasporal inclusions. Southeast Asian J Trop Med Public Health. 39, 235-245 (2008).
  35. FreePDF
  36. Abe, Y., Shimada, H. and Kitada, S. Raft-targeting and oligomerization of Parasporin-2, a Bacillus thuringiensis crystal protein with anti-tumour activity. J. Biochem. 143, 269-275 (2008). Link to PubMed
  37. Lin, Y., Zhang, T., Chen, Z. and Cai, F. The 3-D structure of crystal protein parasporin-2 that is non-hemolytic but capable of preferentially killing cancer cells. Acta Laser Biology Sinica, 17, 181-185 (2008)(in Chinese). Link
  38. Zhu, L., Li, C., Wu, J., Liang, J. and Shi, Y. Apoptosis of HL-60 cells induced by crystal proteins from Bacillus thuringiensis Bt9875. Wei Sheng Wu xue Bao, 48, 690-694 (2008) (in Chinese). Link to PubMed
  39. Okumura, S., Saitoh, H., Ishikawa, T., Mizuki, E. and Inouye, K. Identification and characterization of a novel cytotoxic protein, parasporin-4, produced by Bacillus thuringiensis A1470 strain. Biotechnol. Annu. Rev. 14, 225-252 (2008) Link to PubMed
  40. Nagamatsu, Y., Okamura, S., Saitou, H., Akao, T. and Mizuki, E. Three Cry toxins in two types from Bacillus thuringiensis strain M019 preferentially kill human hepatocyte cancer and uterus cervix cancer cells. Biosci. Biotechnol. Biochem. 74, 494-498 (2010) FreePDF
  41. Shimada, H. and Kitada, S. Mega assemblages of oligomeric aerolysin-like toxins stabilized by toxin-associating membrane proteins. J. Biochem. 149, 103-115 (2011) Link to PubMed
  42. Al-Sayes, F., Azhar, F. and Hindawi, S. Antimalignancy Activity of Bacillus thuringiensis Serovar Dakota (H15) in vivo. World J. Med. Sci. 6, 6-16 (2011) FreePDF
  43. Okumura, S., Saitoh, H., Ishikawa, T., Inouye, K. and Mizuki, E. Mode of action of parasporin-4, a cytocidal protein from Bacillus thuringiensis. Biochim. Biophys. Acta. 1808, 1476-1482 (2011) Link to PubMed
  44. Ekino, K., Okumura, S., Ishikawa, T., Kitada, S., Saitoh, H., Akao, T., Oka, T., Nomura, Y., Ohba, M., Shin, T. and Mizuki, E. Cloning and Characterization of a Unique Cytotoxic Protein Parasporin-5 Produced by Bacillus thuringiensis A1100 Strain. Toxins (Basel) 6, 1882-1895 (2014) FreePDF
  45. Okumura, S., Ishikawa, T., Saitoh, H., Akao, T. and Mizuki, E. Identification of a second cytotoxic protein produced by Bacillus thuringiensis A1470. Biotechnol. Lett. 35, 1889-1894 (2013) Link to PubMe
  46. Kuroda, S., Begum, A., Saga, M., Hirao, A., Mizuki, E., Sakai, H. and Hayakawa, T. Parasporin 1Ac2, a Novel Cytotoxic Crystal Protein Isolated from Bacillus thuringiensis B0462 Strain. Curr Microbiol. 66, 475-480 (2013) Link to PubMe
  47. Okumura, S., Koga, H., Inouye, K. and Mizuki, E. Toxicity of parasporin-4 and health effects of pro-parasporin-4 diet in mice. Toxins (Basel) 2115-2126 (2014) FreePDF
  48. Kuroda S, Begum A, Saga M, Hirao A, Mizuki E, Sakai H, Hayakawa T. Parasporin 1Ac2, a novel cytotoxic crystal protein isolated from Bacillus thuringiensis B0462 strain. Curr Microbiol. 475-480 (2013) Link to PubMed
  49. Okumura, S., Koga, H., Inouye, K. and Mizuki, E. Identification of a second cytotoxic protein produced by Bacillus thuringiensis A1470. Biotechnol Lett. 1889-1894 (2013) Link to PubMed
  50. Ekino K, Okumura S, Ishikawa T, Kitada S, Saitoh H, Akao T, Oka T, Nomura Y, Ohba M, Shin T, Mizuki E. Cloning and characterization of a unique cytotoxic protein parasporin-5 produced by Bacillus thuringiensis A1100 strain. Toxins (Basel) 1882-1895 (2014) FreePDF